Table 2.
Engineering strategies to improve butanol tolerance and production in E. coli
| Butanol tolerance | Butanol production | |||||
|---|---|---|---|---|---|---|
| Strategy | Tolerance (v/v) | Reference | Strategy | Production (mg/l) | Reference | |
| 1 | Overexpression of ompC-tmt | From 1.50 to 2.00% | [28] | thil, crt, bcd, etfA, etfB, hbd, adhe, ompC-tmt | 320 mg/l | This work |
| 2 | N.D. | N.D. | N.D. | atoB, crt, bcd, etfAB, hbd, adhe, ΔadhE, ΔldhA, ΔfrdBC, Δfnr, Δpta | 552 mg/l | [5] |
| 3 | N.D. | N.D. | N.D. | thil, hbd, crt, bcd, etfA, etfB, adhe, high cell density | 1,200 mg/l | [6] |
| 4 | Overexpression of ATF, fabD, feoA and srpABC | From 1.50 to 2.00% | [38] | N.D. | N.D. | N.D. |
| 5 | double disruptions of proV and marR Mutation of lon | From 1.00 to 2.00% | [37] | N.D. | N.D. | N.D. |
| 6 | Overexpression of groESL | From 0.75 to 1.00% | [34] | N.D. | N.D. | N.D. |
| 7 | Mutation of cyclic AMP receptor protein (CRP) | From 0.80 to 1.20% | [33] | N.D. | N.D. | N.D. |
N.D. no data