FIG. 2.

Coimmunoprecipitation of proteins present in the replication compartment. 3T3 cells were transfected with M112/113 or M112/113 and IE3-GFP and then lysed, and their extracts were immunoprecipitated with either anti-M112/113 antibodies or anti-GFP antibodies. Following sodium dodecyl sulfate-polyacrylamide gel electrophoresis of isolated proteins, Western blots were prepared and probed with the antibodies indicated on the left after successive stripping of the same transfer membrane. Lane 1, 5% input; lane 2, extract immunoprecipitated with nonspecific serum control; lane 3, extracts immunoprecipitated with anti-M112/113 antibodies; lane 4, extracts immunoprecipitated with anti-GFP antibodies (IE3). IP, immunoprecipitate.