FIG. 2.

Effect of siRNAs to NSP4 and VP7 on the expression of the corresponding protein in infected cells. MA104 cells transfected with the indicated siRNAs were infected 48 h posttransfection, and at 8 hpi, the cells were fixed and processed for immunofluorescence. The expression levels of VP7 and NSP4 were monitored by using MAbs 60 (α-VP7) and B4 (α-NSP4), respectively, and the cell infection by RRV was monitored by using a rabbit polyclonal antiserum to rotavirus (α-RRV) followed by anti-mouse Alexa-568 and anti-rabbit Alexa-488 antibodies, respectively.