Table 3.
Fatty acid composition of erythrocyte phospholipids from control mice and high-fat diet (HFD) fed mice receiving hydroxytyrosol (HT) supplementation
| Fatty acid composition (g/100 g FAME) | ||||
|---|---|---|---|---|
| Groups | ||||
| Control diet (CD) | High fat diet (HFD) | |||
| Saline | HT | Saline | HT | |
| Fatty acid | (a) | (b) | (c) | (d) |
| C16:0 | 34.5 ± 3.1c | 31.9 ± 2.9c | 41.6 ± 3.8a,b,d | 36.6 ± 3.5c |
| C18:0 | 3.62 ± 0.3c,d | 3.45 ± 0.4c,d | 6.58 ± 0.7a,b | 5.58 ± 0.6a,b |
| C18:1n-9 | 24.6 ± 1.9 | 25.6 ± 2.2 | 22.9 ± 2.3 | 24.8 ± 2.5 |
| C18:2n-6 (LA) | 9.91 ± 1.2 | 10.2 ± 0.9 | 8.66 ± 1.7 | 9.52 ± 1.5 |
| C18:3n-3 (ALA) | 1.21 ± 0.5 | 1.12 ± 0.3 | 1.05 ± 0.3 | 1.05 ± 0.3 |
| C20:4n-6 (AA) | 14.6 ± 1.4 | 15.7 ± 1.6 | 12.6 ± 1.1 | 12.4 ± 1.3 |
| C20:5n-3 (EPA) | 2.05 ± 0.3c | 2.19 ± 0.4c | 0.75 ± 0.03a,b,d | 1.96 ± 0.2c |
| C22:5n-6 (DPAn-6) | 0.16 ± 0.04c | 0.19 ± 0.05c | 0.05 ± 0.01a,b,d | 0.12 ± 0.02c |
| C22:5n-3 (DPAn-3) | 0.78 ± 0.05c | 0.86 ± 0.06c,d | 0.25 ± 0.01a,b,d | 0.70 ± 0.04b,c |
| C22:6n-3 (DHA) | 4.11 ± 0.3c | 4.32 ± 0.4c | 1.98 ± 0.1a,b,d | 3.98 ± 0.3c |
| Total SFAs | 38.8 ± 2.9c | 36.5 ± 2.6c,d | 49.7 ± 4.4a,b | 43.5 ± 3.9b |
| Total MUFAs | 26.4 ± 2.5 | 27.9 ± 2.4 | 24.5 ± 2.2 | 24.8 ± 2.7 |
| Total PUFAs | 34.8 ± 3.3c | 35.6 ± 3.6c | 25.8 ± 2.2a,b | 31.7 ± 2.6 |
| Total LCPUFAs | 22.9 ± 1.7c | 23.7 ± 2.1c | 15.8 ± 1.1a,b,d | 20.2 ± 1.5c |
| Total n-6 LCPUFAs | 14.9 ± 1.4 | 16.0 ± 1.6c | 12.7 ± 0.9b | 12.7 ± 1.4 |
| Total n-3 LCPUFAs | 8.00 ± 0.7c | 7.70 ± 0.5c | 3.10 ± 0.2a,b,d | 7.50 ± 0.6c |
| n-6/n-3 LCPUFA ratio | 1.86 ± 0.2c | 2.07 ± 0.3c | 4.10 ± 0.4a,b,d | 1.69 ± 0.3c |
Values are expressed as g fatty acid per 100 g FAME and represent the mean ± SEM for n = 12–14 mice per experimental group. Significant differences between the groups are indicated by the letter identifying each group (one-way ANOVA and Newman-Keuls´ post-test; p < 0.05). The identification of saturated and unsaturated fatty acids and their relationships are shown in Table 2