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. 2005 Jan;79(1):95–105. doi: 10.1128/JVI.79.1.95-105.2005

FIG. 3.

FIG. 3.

The distal region of the relB promoter contains an AP-1 binding site. (A) NIH 3T3 cell nuclear extracts (5 μg) were subjected to competition EMSA by using the oligonucleotide containing the consensus AP-1 element as a probe and 10- or 30-fold excess unlabeled oligonucleotides containing either the consensus (Con) AP-1 element or the putative AP-1 element Puta-AP-1(1) or Puta-AP-1(2), as indicated. (B) NIH 3T3 cell nuclear extracts (5 μg) were subjected to competition EMSA by using either the consensus AP-1 element, Puta-AP-1(1), or Puta-AP-1(2) as a probe, and 10- or 30-fold excess unlabeled consensus AP-1 oligonucleotide. ns, Position of non-AP-1 band.