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CMV IE1 protein activates c-Jun phosphorylation. NIH 3T3 cells were transfected with 10 μg of pON2205 vector expressing IE1 protein (IE1) or pCMV empty vector DNA (EV). After 4 h, cells were treated with DMSO, 20 μM SP600125, or 40 μM SP600125 for 20 h. Total cell lysates were prepared, and samples (30 μg) were subjected to immunoblot analysis for IE1, c-Jun, phospho-Ser63 c-Jun (P-c-Jun), c-Fos, Fra-2, JunB, JunD, and β-actin (as a loading control).
