FIG. 6.

Evidence for a kissing-loop interaction between the loops of 5BSL3.2 and SL2 in the X-tail sequence. (A) Predicted secondary structures of 5BSL3.2 and X-tail SL2, with complementary nucleotides in the loop regions indicated by connecting lines. (B) The nucleotide sequences of the complementary loop regions in 5BSL3.2 and X-tail SL2 are depicted in the middle panel. Noncomplementary nucleotides are indicated in bold letters. A representative result from a transient replication assay is shown in the lower panel. Mutations specified in the upper panel were introduced into the replicon PI-ins3.1-3 and analyzed for transient replication as described in the legend to Fig. 1. The basic replicon used for this analysis is depicted at the top.