Table 3.

Kinetic parameters of the studied NADP‐GDHs

Enzyme	pH 7.5							pH 5.8
	k cat (s−1)	K m‐α‐KG (mmol/L)	n H‐ α‐KG	K m‐NADPH (μmol/L)	n H‐NADPH	K m‐NH4+ (mmol/L)	n H‐NH4+	k cat (s−1)	K m‐ α‐KG (mmol/L)	n H‐ α‐KG
ScGdh1	13	0.37		45		8.6		12	0.31
ScGdh3	14	0.4		42		6.9		13	S 0.5=1.48	2.4
LkGdh1	20	0.46		46		7.4		10	0.34
KlGdh1	21	S 0.5=3.61	4.4	S 0.5=39	1.8	S 0.5=21.4	2.7	6	S 0.5=1.95	4

Data were fitted to the Michaelis–Menten equation except for the ScGdh3 isoform at pH 5.8 and the KlGdh1 enzyme which were fitted to the Hill equation. Standard errors for the fitted parameters at pH 7.5 were lower than 8% and, at pH 5.8, lower than 16%. For all the fitting analyses, the R ² value was higher than 0.982.