Table 4.
Hierarchical analysis of molecular variance (AMOVA) among human clinical sources of Giardia duodenalis belonging to genetic assemblages A and B
Gene | df | Sum of squares | Components of variance | Percentage of variation | p‐value | Fixation index | Significant FST | |
---|---|---|---|---|---|---|---|---|
Genetic assemblage A HC × DC | ||||||||
Among populations | tpi | 1 | 0.93 | 0.045 | 7.53 | |||
Within populations | tpi | 23 | 12.95 | 0.563 | 92.47 | |||
Total | 24 | 13.88 | 0.608 | 100 | 0.239 | 0.075 | – | |
Genetic assemblage A HC × DC | ||||||||
Among populations | gdh | 1 | 0.774 | 0 | 0 | |||
Within populations | gdh | 26 | 22.333 | 0.858 | 100 | |||
Total | 27 | 23.107 | 0.858 | 100 | 0.332 | 0 | – | |
Genetic assemblage B HC × DC | ||||||||
Among populations | tpi | 1 | 1.504 | 0 | 0 | |||
Within populations | tpi | 42 | 71.542 | 1.703 | 100 | |||
Total | 43 | 73.046 | 1.703 | 100 | 0.419 | 0 | – | |
Genetic assemblage B HC × DC | ||||||||
Among populations | gdh | 1 | 4.751 | 0.253 | 15.75 | |||
Within populations | gdh | 26 | 35.321 | 1.358 | 84.25 | |||
Total | 27 | 40.072 | 1.612 | 100 | 0.0005 | 0.157 | + |
HC, hospital group; DC, daycare group; –, indicates that these groups are genetically similar according to the AMOVA test; +, indicates that these groups are genetically different according to the AMOVA test.