Table 2.
CSF flow cytometry characterization of cancer floating cells in 13 cases of breast cancer leptomeningeal metastasis
| Case number | CSF cellularity (cells/μl) | Number of events analyzed | Percentage of BC cells (CD45-negative) | Percentage of positive cells within the CD45-negative BC population | |||||
|---|---|---|---|---|---|---|---|---|---|
| CD15% | CD138% | CD227% | CD24% | CD44% | CD133% | ||||
| 1 | 32 | 181,000 | 9 | nd | 82 | nd | nd | nd | nd |
| 2 | 1 | 2887 | 47 | nd | 99 | nd | nd | nd | nd |
| 3 | 86 | 132,260 | 43 | 24 | 96 | nd | 98 | 94 | 96 |
| 4 | 66 | 12,817 | 96 | 51 | 97 | nd | nd | nd | neg |
| 5 | 2 | 6078 | 4 | 75 | 80 | nd | nd | nd | 93 |
| 6 | 50 | 45,134 | 20 | neg | 91 | 93 | 94 | 97 | neg |
| 7 | 1 | 4612 | 20 | 66 | 54 | 97 | 90 | 88 | 59 |
| 8 | 2 | 1183 | 35 | nd | 80 | 94 | nd | 87 | 80 |
| 9 | 30 | 24,300 | 47 | neg | 56 | 82 | neg | neg | neg |
| 10 | 17 | 29,798 | 40 | 92 | 98 | 96 | 77 | 96 | neg |
| 11 | 1 | 1293 | 60 | 12 | 93 | 96 | 100 | 99 | neg |
| 12 | 8 | 3550 | 45 | 98 | 89 | 97 | neg | 56 | neg |
| 13 | 8 | 8455 | 36 | neg | 66 | 88 | 89 | neg | 88 |
Breast cancer marker expression is reported as the percentage of positive cells within the CD45-negative/side-scatter large population
CSF cerebrospinal fluid, BC breast cancer, nd not done, neg negative