TABLE 2.
Cells from multiple species that are resistant to ENTV vector transduction become susceptible after expression of human Hyal2a
| Target cell type | Expressed protein | Apparent titer of LAPSN vector (AP+ foci/ml) produced by packaging cells:
|
Ratio of JSRV to ENTV vector titers | |
|---|---|---|---|---|
| PN229 (ENTV) | PJ4 (JSRV) | |||
| SSF | None | 4 × 105 | 4 × 105 | 1 |
| HT-1080 | None | 200 | 2 × 104 | 100 |
| Hyal2 | 1 × 105 | 4 × 105 | 4 | |
| D17 | None | <5 | 7 × 103 | >1,400 |
| Hyal2 | 1 × 106 | 3 × 105 | 0.3 | |
| Vero | None | <5 | 2 × 104 | >4,000 |
| Hyal2 | 1 × 105 | 9 × 104 | 0.9 | |
| NIH 3T3 | None | 10 | <5 | <0.5 |
| Hyal2 | 6 × 104 | 5 × 104 | 0.8 | |
| 208F | None | <1 | <1 | |
| Hyal2 | 400 | 3 × 104 | 75 | |
| oHyal2b | 200 | 6 × 103 | 30 | |
| rHyal2 | <1 | 9 × 103 | >9,000 | |
Cells expressing human or rat Hyal2 were generated by transduction with vectors encoding these proteins and Neo (LHyal2SN and LrHyal2SN, respectively) followed by selection in G418. LAPSN vector titers were determined by infection of cells seeded the day before at 105 cells per well (diameter, 3.5 cm) of 6-well plates. AP+ foci were counted 3 days after vector exposure. Results are means of two to five experiments each, and experimental values varied by no more than four-fold from the mean.
Cells expressing ovine Hyal2 were generated by transfection with a plasmid that expresses ovine Hyal2 and Neo (pCR3.1oHyal2) followed by selection in G418.