TABLE 3.
Multiple rat cell lines are resistant to ENTV vector transduction even after expression of human Hyal2 in the cellsa
| Target cell type | Expressed protein | Apparent titer of LAPSN vector (AP+ foci/ml) produced by packaging cells:
|
Ratio of JSRV to ENTV titers | |
|---|---|---|---|---|
| PN229 (ENTV) | PJ4 (JSRV) | |||
| Rat2 | Hyal1 | <1 | <5 | |
| Hyal2 | 20 | 2 × 104 | 1,000 | |
| NRK | Hyal1 | <1 | <5 | |
| Hyal2 | 20 | 8 × 103 | 400 | |
| XC | Hyal1 | <1 | <5 | |
| Hyal2 | 14 | 3 × 103 | 200 | |
| 9L | Hyal1 | <1 | <5 | |
| Hyal2 | 7 | 1 × 103 | 140 | |
a
Cells expressing human Hyal1 or human Hyal2 were generated by transduction with vectors encoding these proteins and Neo (LHyal1SN and LHyal2SN, respectively) followed by selection in G418. LAPSN vector titers were determined by infection of cells seeded the day before at 105 cells per well (diameter, 3.5 cm) of 6-well plates. AP+ foci were counted 3 days after vector exposure. Results are means of at least two experiments. Values from different experiments varied by no more than three-fold.