TABLE 4.
ENTV vector transduction of D17 dog cells is unaffected by coculture with 208F rat cellsa
| No. of cells (× 104) seeded
|
Apparent titer (AP+ foci/ml) of ENTV vector
|
||
|---|---|---|---|
| D17/Hyal2 | 208F/Hyal2 | Expt 1 | Expt 2 |
| 5.0 | 0.0 | 1.1 × 105 | 1.8 × 105 |
| 2.5 | 2.5 | 4.5 × 104 | 9.0 × 104 |
| 0.0 | 5.0 | 200 | 180 |
a
D17 and 208F cells that express human Hyal2 were seeded at the indicated numbers into wells of 6-well plates. The next day the cells were exposed to LAPSN vector made from PN229 ENTV pseudotype packaging cells, and 2 days after vector exposure the cells were stained for AP. Results are means of two wells for experiment 1 and of 4 wells for experiment 2.