FIG. 3.

Plaque phenotypes of gB-, gK-, and UL20-null viruses. The plaque phenotypes of YEbac102, YEbacΔUL20, YEbacΔgB, and YEbacΔgK were observed on Vero (A1, B1, C1, and D1), Fd20-1 (A2, B2, C2, and D2), D6 (A3, B3, C3, and D3), and FcgK-1 (A4, B4, C4, and D4) cells. Confluent cell monolayers were infected with wild-type YEbac102 (A1, A2, A3, and A4), YEbacΔUL20 (B1, B2, B3, and B4), YEbacΔgB (C1, C2, C3, and C4), or YEbacΔgK (D1, D2, D3, and D4) at an MOI of 0.001, and viral plaques were visualized at 48 h postinfection by immunohistochemistry.