Plaque phenotypes of double-null viruses and rescued viruses. The plaque phenotypes of YEbacΔgBΔgUL20 and YEbacΔgBΔgK on Vero (A1 and B1), Fd20-1 (A2 and B2), D6 (A3 and B3), and FcgK-1 (A4 and B4) cells or Ad5-gB infected Fd20-1 (A5 and B5) and FcgK-1 (A6 and B6) cells are shown. Confluent cell monolayers were infected with YEbacΔgBΔgUL20 (A1, A2, A3, A4, A5, and A6) or YEbacΔgBΔgK (B1, B2, B3, B4, B5, and B6) at an MOI of 0.001. The plaque phenotypes of the rescued gB-, gK-, and UL20-null (D, E, and F) and double-null (G and H) viruses in comparison to the wild-type YEbac102 (C) on Vero cells are shown. Viral plaques were visualized at 48 h postinfection by immunohistochemistry.