TABLE 1.

Titers of packaged replicons after passaging at different MOIs^a

Replicon (MOI)	RNA cotransfection	Passage no.:
		2	3	4	5
SINrep/LacZ (10 inf.u/cell)	3.5 × 108	6.2 × 108	2.7 × 108	3.2 × 108	1.1 × 107
SINrep/LacZ (2 inf.u/cell)	3.5 × 108	1.3 × 109	1.5 × 109	3.3 × 108	3.4 × 108
SINrep/GFP (10 inf.u/cell)	3.6 × 108	3.7 × 108	2.5 × 108	3.3 × 108	3.6 × 108
SINrep/GFP (2 inf.u/cell)	3.6 × 108	1.1 × 109	2.3 × 109	4.0 × 108	2.1 × 109

^ainf.u, infectious unit.