Fig. 2.

c-Myc regulates the RBM38 expression by binding to the promoter region of RBM38 gene in breast cancer cells. a–d MCF-7 and ZR-75-1 cells were transfected with two siRNAs to knockdown c-Myc (siRNA1, siRNA2) and the control (CTRi). RBM38 expression was obviously upregulated at both the protein (a, b) and mRNA levels (c, d) following c-Myc knockdown. e Schematic diagram of the luciferase reporter constructs containing the E-box in promoter region of RBM38 gene. f MCF-7 and g ZR-75-1 cells with c-Myc knockdown siRNA and the control were transfected with pGL3 control reporter or pGL3 reporter carrying the E-box in promotor region of RBM38 gene. The fold increased in relative luciferase activity was a product of the luciferase activity induced by c-Myc knockdown divided by that induced by the control. Results were representative of three independent experiments and presented as the mean ± SEM. *P < 0.05. h c-Myc could directly bind to E-box in promoter region of RBM38 gene. Lane 1, Input DNA; Lane 2, DNA from MCF-7 cells immunoprecipitated with normal mouse IgG; Lane 3, DNA from MCF-7 cells immunoprecipitated with anti-c-Myc antibody