FIG. 1.

AAV2 Rep proteins inhibit Ad5 replication. (A) β-Gal activity was measured from HeLa cell extracts obtained after transfection with pCDM8 plasmids expressing Rep proteins and infected with Ad5lacZ5. β-Gal activity was normalized to the pCDM8 vector control (not shown). Error bars represent the standard deviations from four experiments performed in triplicate (n = 12). (B) Slot blot hybridization analysis on nuclear extracts from HeLa cells with radiolabeled Ad5 probe. HeLa cells were transfected with either pCDM8 (slots 1 and 3) or pCDMRep78 (slots 2 and 4). Input slots contain equal amounts of chromatin, as determined by the absorbance at 260 nm. CHIP slots contain chromatin-immunoprecipitated DNA. Slots 5 to 8 contain decreasing amounts of the Ad5 genome excised from plasmid pJM17. The radioactive signal in slots 5 to 8 was measured with a phosphorimager, and the amount of DNA in slots 1 to 4 was determined by comparison to the known amounts of DNA in slots 5 to 8.