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. 2005 Jan;79(1):28–38. doi: 10.1128/JVI.79.1.28-38.2005

FIG. 2.

FIG. 2.

Rep78 protein is preferentially cross-linked at the Ad5 E2a promoter region. (A) CHIP assay from nuclear lysates of Ad5-infected cells transfected with pCDMRep78. Input DNA (before immunoprecipitation, diluted 1:1,000) and CHIP DNA (after immunoprecipitation, 1 μl) were amplified by using Ad5 promoter region primers. PCR products were separated by agarose gel electrophoresis and stained with ethidium bromide. Lanes labeled “+” were from cells transfected with pCDMRep78; lanes labeled “−” were from cells transfected with pCDM8. The sizes of the largest and smallest products are indicated at the left side of the gel. Equal amounts of protein were compared from nuclear lysates for the expression of E2a (B) and Rep78 (C). Lysates were from Ad-infected HeLa cells transfected with pCDM8 (lanes 1 and 3) or pCDMRep78 (lanes 3 and 4). Lane 5 in panel C contains a positive control for the Rep78 protein.