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. 2005 Jan;79(1):28–38. doi: 10.1128/JVI.79.1.28-38.2005

FIG. 5.

FIG. 5.

Rep68 protects a region of the Ad5 E2a promoter from DNase I digestion. (A) DNase I protection assays were performed on the 303-bp fragment (∼600 pmol) containing labeled lower strand (lanes 1 to 4) or labeled upper strand (lanes 5 to 8) with decreasing concentrations of Rep68. (B) DNase I protection assays were performed on labeled upper strand in the presence of Rep68, RepNT, and Rep40. Radiolabeled DNA size markers (not shown) were used to determine the corresponding nucleotide position in the Ad5 genome.