Figure 5. Sedimentation Equilibrium SEDFIT-MSTAR output for analysis of VanS in HGN buffer pH~7.9, I = 0.1, (containing glycerol) at 7.0 °C at a loading concentration of 0.326 mg mL⁻¹ in long (20 mm) path length cells.

(a) concentration (fringe displacement units versus radial displacement from the centre of rotation, r (b) log concentration versus the square of the radial displacement (c) extrapolation of the M* function to the cell base to yield the “whole distribution” weight average molar mass M_w, = (47.5 ± 1.0) kDa; (d) plot of the point average molar mass (local molar mass) – obtained by taking the derivative of the data from plot (b) versus local concentration c(r) in the analytical ultracentrifuge cell. The value at the hinge point (where c(r) = the cell loading concentration, yields another estimate for the whole distribution M_w ~ (46.4 ± 1.0) kDa.