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. 2017 Apr 11;7:46177. doi: 10.1038/srep46177

Figure 5. Capability of maturation of second passage cells grown with Matrigel.

Figure 5

Second passage cells were cultured for 14 days and then treated with Matrigel for 7 days. (A) The gene expression of Alb, Hnf4a, Cebpa, Tat, Tdo2, and Cyp2b1 was measured by qPCR. The second passage cells were cultured for 21 days [Mat(−)], and a subset of cells was treated with Matrigel from day 14 to day 21 [Mat(+)]. MH: isolated hepatocytes from a normal adult liver; PrSH: CD44+ SH. *p < 0.05. (B) Albumin secretion by second passage cells was measured using ELISA. The second passage cells were cultured for 21 days [Mat(−)], and a subset of cells was treated with Matrigel from day 14 to day 21 [Mat( + )]. *p < 0.05. (C) CYP3A activity was measured in second passage cells treated without [Mat(−)] or with Matrigel [Mat(+)]. *p < 0.005. (D) To examine the ability of cells to accumulate glycogen, PAS staining was performed in cells without [Mat(−)] or with Matrigel [Mat(+)]. Scale bar = 100 μm.