Figure 10. Analysis of gpatch3 overexpression-associated phenotypes in histological transverse sections of zebrafish.

Gpatch3 mRNA microinjected zebrafish embryos were analyzed at 96 hpf and two phenotypes (Ph-A and Ph-B) with increasing severity were described. Brightfield micrographs of 10 μm cryostat transverse head sections stained with hematoxilin and eosin show altered phenotypes including microphthalmia, iridocorneal angle defects, periocular and intracraneal edemas (asterisks) and underdevelopment of pharyngeal cartilages (arrowhead). Scale bars represent 100 μm in head section panels and 20 μm in iridocorneal angle magnification panels. Xa: xantophores. Ir: iridophores. M: periocular mesenchyme.