Figure 8. Analysis of gpatch3 knock-down-associated phenotypes in histological transverse sections of zebrafish gpatch3 morphants.

Gpatch3^ATG MO-injected zebrafish embryos were analyzed at 96 hpf and four phenotypes (Ph-1-4) with increasing severity were described. The 100X and 400X brightfield micrographs of 10 μm cryostat transverse head sections stained with hematoxilin and eosin show altered phenotypes including microphthalmia, mandibular maldevelopment (asterisks), enlarged intracranial and periocular spaces (empty arrowheads) and iridocorneal angle defects (empty arrows). Scale bars represent 100 μm in head section panels and 20 μm in iridocorneal angle magnification panels. Xa: xantophores. Ir: iridophores. M: periocular mesenchyme.