FIG. 1.

Compounds affecting Py infectivity on R− cells. R− cells supplemented with GD1a were treated with MBCD (methyl-β-cyclodextrin), nystatin, latrunculin A, jasplakinolide, demecolcine, or taxol for 1 h at 37°C prior to infection. Cells were infected at an MOI of ∼500 in the presence or absence of these compounds, as indicated. Infected cells were processed and analyzed for Py LT antigen (LTAg) by indirect immunofluorescence. Each individual experiment was performed three times in triplicate. The data presented are an average of the three experiments.