FIG. 4.

Comparison of proteins from immunoprecipitated ts1201 procapsids with those of wt HSV-1 strain 17 B capsids. Proteins of immunoprecipitated procapsids from ts1201-infected cells (lane 5) and purified wt virus B capsids (lane 6) were analyzed by SDS-PAGE, and the separated proteins were detected by staining with Coomassie brilliant blue. As a control, an extract of ts1201-infected cells was treated in the same way as that described for the isolation of procapsids, except no VP5 antibody was added (lane 4). In addition, extracts of cells that had been infected with the VP19C deletion mutant, vΔ38YFP, under the same conditions as those for ts1201 were also treated with VP5 monoclonal antibody (Mab), using the procapsid isolation protocol (lane 3). Purified 6F10 and DM165 monoclonal antibodies were analyzed in lanes 1 and 2, respectively. Protein size markers (in kilodaltons), indicated by the short lines, are shown at the far left-hand side of the panel. The HSV-1 capsid proteins are indicated by the closed triangles. The presence (+) or absence (−) of monoclonal antibody in the immunoprecipitation reaction is indicated at the top of the image of the gel.