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. 2005 Jan;25(1):364–376. doi: 10.1128/MCB.25.1.364-376.2005

FIG. 2.

FIG. 2.

Modulation of myocardin transcriptional activity by p300 and HDAC5. (A) COS cells were transiently transfected with expression vectors encoding myocardin (0.1 μg) and p300 (0.5 and 1 μg) and the indicated luciferase reporters as described in Materials and Methods. (B) COS cells were transiently transfected with expression vectors encoding the GAL4 DNA-binding domain fused to amino acids 1 to 935 (GAL4-Myo) of myocardin (left graph) or 713 to 935 (GAL4-Myo-TAD) of myocardin, which encompasses the TAD (right graph) and p300 (1 μg) and a UAS-luciferase reporter. (C) COS cells were transiently transfected with expression vectors encoding myocardin and HDACs (0.1, 0.2, and 0.5 μg) and the indicated luciferase reporters. (D) COS cells were transiently transfected with expression vectors encoding the GAL4 DNA-binding domain fused to amino acids 1 to 935 (GAL4-Myo) of myocardin, HDAC5 (0.1 and 0.5 μg), or HDAC1 (0.1 and 0.5 μg) and a UAS-luciferase reporter. (E) COS cells were transiently transfected with expression vectors encoding myocardin (0.2 μg), p300 (1 μg), HDAC5 (0.5 μg), and increasing amounts of p300 (0.5, 1, 2, and 4 μg) and HDAC5 (0.5, 1, and 2 μg), respectively, as indicated, and the SM22-luciferase reporter.