FIG. 6.

Blockade of myocardin-induced smooth muscle gene expression by E1A. (A) 10T1/2 cells were transiently transfected with expression vectors encoding myocardin and E1A and an SM22-luciferase reporter (left part) or GAL4-myocardin and E1A and a UAS-luciferase reporter (right part), as indicated. (B) 10T1/2 cells were transiently transfected with expression vectors encoding myocardin and E1A, as indicated, and cells were stained for expression of SM α-actin. A representative cell expressing SM α-actin is shown (left part), as is blockade of myocardin-induced SM α-actin expression by E1A (right part). Values are expressed as the relative number of positive cells compared to the number in cultures transfected with myocardin alone, which was assigned a value of 100.