Table-1.

Components of IVM media used in the experiment 2.

S. No.	IVM media groups	Composition of different IVM media
1	G1	Control medium: TCM 199B+Gentamicin (50 µg/ml)
2	G2	Control medium+KP: 10 µg/ml
3	G3	Control medium+FSH: 10 µg/ml
4	G4	Control medium+FSH (10 µg/ml)+KP (10 µg/ml)
5	G5	Control medium+LH: 10 µg/ml
6	G6	Control medium+LH (10 µg/ml)+KP (10 µg/ml)
7	G7	Control medium+E2: 1 µg/ml
8	G8	Control medium+E2 (1 µg/ml)+KP (10 µg/ml)
9	G9	Control medium+FSH (10 µg/ml)+LH (10 µg/ml)+E2 (1 µg/ml)
10	G10	Control medium+FSH (10 µg/ml)+LH (10 µg/ml)+E2 (1 µg/ml)+KP (10 µg/ml)
11	G11	Control medium+FSH (10 µg/ml)+LH (10 µg/ml)+E2 (1 µg/ml)+FBS (10%)
12	G12	Control medium+FSH (10 µg/ml)+LH (10 µg/ml)+E2 (1 µg/ml)+FBS (10%)+KP (10 µg/ml)

IVM=In vitro maturation, KP=Kisspeptin, FSH=Follicle stimulating hormone, LH=Luteinizing hormone, E₂=Estradiol, FBS=Fetal bovine serum