Table-3.
Effect of KP in combination with FSH, LH, and E2 on in vitro maturation of sheep oocytes.
| Groups | Combinations | Number of oocytes (replicates) | Proportion of CCE | Proportion of extrusion of 1st PB |
|---|---|---|---|---|
| G1 | Control | 120 (6) | 69.17±2.71b | 6.67±1.05a |
| G2 | KP-10 | 120 (6) | 76.67±2.47c | 26.67±2.11b |
| G3 | FSH | 120 (6) | 91.67±1.67e | 54.17±2.71c |
| G4 | FSH+KP | 120 (6) | 94.17±0.83ef | 69.17±3.27e |
| G5 | LH | 120 (6) | 84.17±1.54d | 58.33±4.01cd |
| G6 | LH+KP | 120 (6) | 89.17±3.00de | 64.17±3.01de |
| G7 | E2 | 120 (6) | 52.50±2.14a | 31.67±2.11b |
| G8 | E2+KP | 120 (6) | 67.50±1.71b | 59.17±2.39cd |
| G9 | FSH+LH+E2 | 120 (6) | 90.83±2.01e | 60.01±2.58cd |
| G10 | FSH+LH+E2+KP | 120 (6) | 95.01±0.01ef | 70.01±1.29e |
| G11 | FSH+LH+E2+FBS | 120 (6) | 95.01±0.01ef | 80.00±1.29f |
| G12 | FSH+LH+E2+FBS+KP | 120 (6) | 98.33±1.05f | 89.17±2.38g |
Means with different superscripts, within a column, are significantly different. One-way ANOVA followed by Duncan’s multiple range test (p<0.05). FSH=Follicle stimulating hormone, LH=Luteinizing hormone, E2=Estradiol, KP=Kisspeptin, CCE=Cumulus cell expansion, PB=Polar body, FBS=Fetal bovine serum