Fig. 2.

Bland–Altman plots showing relationship between fresh and frozen IFNγ ELISpot responses from individual samples. Mean SFU/million PBMCs of combined fresh and frozen data for each sample is plotted against the difference between the same data for each peptide. Data shown is for days 63 and C-1 (day 76 (−4/+3)) combined. Dashed line indicates mean difference for all samples. Dotted line in TRAP and CSP plots represents theoretical threshold where measurable magnitudes from fresh samples are reduced to zero in frozen samples. Reduction in magnitude of TRAP and CSP specific IFNγ response after cryopreservation increases proportionally with overall magnitude of fresh response.