Fig. 4.

ICS flow cytometry data for paired samples stimulated with TRAP peptide megapool in (a) CD3⁺CD4⁺ and (b) CD3⁺CD8⁺ populations. Highly significant reductions in the frequency of cells producing either IFNγ, IL-2 or TNFα are observed in the CD3⁺CD4⁺ compartment (Wilcoxon matched pairs signed rank test) however this observation is restricted to Day 63 samples only. A less significant reduction in the frequency of CD3⁺CD8⁺ cells producing cytokine is only observed with IFNγ production at Day 63.