Fig. 5.

Analysis of polyfunctional cytokine response profile TRAP peptide megapool at Day 63 with respect to IFNγ TNFα and IL-2 production. Graphs: all possible combinations of responses for the cytokines analysed are shown on the x-axis with the percentage of those distinct functional cell populations within either (a) CD3⁺CD4⁺ or (b) CD3⁺CD8⁺ responding cells shown on the y-axis. Bars correspond to mean percentages for each functional population. Crosses indicate significant differences between fresh and frozen frequencies for each functional cytokine combination obtained using students t-test. Piecharts: summarises the data by presenting mean frequencies of each functional population as a proportion of combined cytokine production. Segment colour matches the colour code for each cytokine combination on the x-axis of the graphs. Colour coded arcs indicate the mean proportion of total analysed cytokine production for IFNγ (red) TNFα (dark green) and IL-2 (light green). A significant reduction in the frequency of cytokine-producing T cells was observed in the CD4⁺, but not CD8⁺ T cell compartments. The greatest loss was within CD4⁺ T cells populations expressing IFNγ alone or in combination with either IL-2 or TNFα.