Table 1.
Comparison of ELISpot responses. Mean SFU/million PBMC values are provided with 5th and 95th CI of the mean. P-values are for Wilcoxon-matched pairs signed rank test (two–tailed). Comparing fresh with post thaw response in each peptide pool/mitogen response and at each timepoint. Comparisons in bold are statistically significant. n indicates number of paired fresh/frozen datapoints analysed from individual samples.
| Antigen | ELISpot responses |
|||
|---|---|---|---|---|
| Day 0 | Day 63 | C-1 (Day 76 −4/+3) | ||
| Mock | sfu/106 Fresh | 15 (8, 21) | 22 (14, 30) | 21 (14, 27) |
| sfu/106 Frozen | 18 (13, 23) | 12 (5, 20) | 17 (12, 23) | |
| n | 19 | 14 | 21 | |
| p | 0.4118 | 0.0942 | 0.5005 | |
| PHA | sfu/106 Fresh | 281 (177, 385) | 1199 (876, 1522) | 845 (695, 995) |
| sfu/106 Frozen | 1994 (1728, 2259) | 2090 (1835, 2344) | 2140 (1888, 2393) | |
| n | 19 | 14 | 21 | |
| p | <0.0001 | 0.0001 | <0.0001 | |
| CMV | sfu/106 Fresh | 110 (−74, 294) | 149 (−80, 378) | 327 (62, 592) |
| sfu/106 Frozen | 128 (−54, 311) | 127 (−108, 363) | 321 (65, 576) | |
| n | 15 | 13 | 21 | |
| p | 0.9453 | 0.2510 | 0.8500 | |
| TRAP | sfu/106 Fresh | 18 (9, 26) | 650 (491, 811) | 354 (170, 538) |
| sfu/106 Frozen | 17 (6, 27) | 174 (118, 230) | 146 (68, 224) | |
| n | 19 | 14 | 11 | |
| p | 0.8999 | 0.0001 | 0.0010 | |
| CSP | sfu/106 Fresh | 19 (1, 36) | 196 (98, 293) | 91 (68, 115) |
| sfu/106 Frozen | 16 (9, 23) | 27 (17, 38) | 34 (21, 47) | |
| n | 13 | 12 | 19 | |
| p | 0.6355 | 0.0005 | <0.0001 | |
| Pre-freeze viability (%) | 97.7% (97.0, 98.5) | 98.1% (97.7, 98.5) | 98.1% (97.7, 98.5) | |
| Post-thaw Viability (%) | 97.5% (96.4, 98.5) | 97.6% (97.3, 97.8) | 95.3% (93.7, 97.0) | |