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. 2017 Feb 23;7(4):2471–2485. doi: 10.1021/acscatal.6b03182

Figure 9.

Figure 9

Pathway for interconversion between Nia-L and Nia-SI, demonstrated by photolysis measurements. (A) Scheme showing Nia-SI formation from Nia-L, only in the presence of an oxidized proximal cluster. (B) Photolysis spectra of the [NiFe] hydrogenase from D. vulgaris MF under a H2 atmosphere show “normal” formation of Nia-L. (C) Photolysis spectra of [NiFe] hydrogenase from D. vulgaris MF recorded under a N2 atmosphere show formation of both Nia-L and Nia-SI, under conditions where the proximal cluster is at least partially oxidized. Panels (B) and (C) adapted with permission from ref (60). Copyright 2014 Wiley-VCH.