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. 2017 Apr 11;7:46352. doi: 10.1038/srep46352

Figure 4. Compound 6b inhibited STAT3 activity.

Figure 4

(A) Western blot analysis of p-STAT3 (Tyr705), p-STAT3 (Ser727) and STAT3 in whole-cell lysates of equal total protein prepared from MCF-7 and MCF-7/DOX cells treated with compound 6b for 24 h. (B) EMSA analysis of nuclear extracts of equal total protein containing activated STAT3 from MCF-7 cells treated with compound 6b for 24 h after incubation with hSIE probe that binds STAT3. (C) MCF-7 cells were starved overnight followed by treatment with 6b or DMSO for 6 h. Cells were then treated with 50 ng/ml of IL-6 for 30 min. For immunofluorescent assay, cells were fixed and stained with anti-phospho-STAT3 (p-STAT3) and DAPI before subject to ImageXpress Micro Confocal analysis. Red: p-STAT3; blue: nuclei. (D) Western blot analysis of Bcl-2, Bax and Cyclin D1 in MCF-7 and MCF-7/DOX cells treated with compound 6b for 24 h.