Figure 6. Compound 6b induced MCF-7, MCF-7/DOX but MCF-10A cells apoptosis in Vitro with curcumin as reference.
Western blot analysis of Cleaved-PARP, Cleaved Caspase-3 and Cleaved Caspase-9 levels in whole-cell lysates. Annexin V-APC/7-AAD staining was carried out and the percentages of apoptotic cells were further determined using flow cytometry MCF-7 (A), MCF-7/DOX (B) and MCF-10A (D) cells were incubated with 6b at different concentrations for 24 h with curcumin (C) as reference. **p < 0.01, ***p < 0.001.