Skip to main content
. 2005 Jan;25(1):100–113. doi: 10.1128/MCB.25.1.100-113.2005

FIG. 7.

FIG. 7.

DAF-12 and EGL-15 protein levels are reduced in ife-4(ok320) animals. (A) Expression of DAF-12 and EGL-15 was detected by Western blotting at the indicated developmental stages (L1 to L4) for N2 (N) and ife-4(ok320) (I) animals. Proteins (50 μg) were resolved by SDS-PAGE on 10% gels. (B) DAF-12 and EGL-15 protein levels were normalized with the actin signal, and the ife-4(ok320)/N2 ratios are graphically represented, with 1.0 shown by the dashed line. The results represent the average for three independent worm populations and immunoblots.