Figure 8.

Anxiolytic effects of single herbs blocked by the GABA_A receptor BZ-site antagonist flumazenil. Mice were treated with saline vehicle (Veh), single-herb extract (120 mg/kg of oral B, Y, P, or A) with or without flumazenil (namely F, 1.25 mg/kg i.p). Anxiolytic effect was evaluated based on time spent in open arms. Data represent mean ± SD. ^###(p < 0.001) indicates significant difference between test group receiving single herb without flumazenil and Veh group, whereas ^∗∗∗(p < 0.001) indicates significant difference between test group receiving single herb with flumazenil and test group receiving single herb without flumazenil, based on one-way ANOVA test followed by Newman-Keuls test.