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. 2005 Jan;25(1):264–277. doi: 10.1128/MCB.25.1.264-277.2005

FIG. 6.

FIG. 6.

Roscovitine inhibits recruitment of SRC-1 to the MMTV promoter and the acetylation of histone H4 at lysine 5. T47D cells stably transfected with MMTV-CAT were treated with 10 nM R5020 for 30 min or pretreated with 30 μM roscovitine for 3 h and then treated with R5020 for 30 min, and ChIP assays performed using antibodies to PR (A), cyclin A (B), SRC-1 (C), histone H4 acetyl lysine 5 (D), or TRAP-220 (E). In panels A through E the ratio of signal to input was determined and fold enhancement relative to an assigned value of 1 for vehicle-treated samples is plotted. (F) SRC-1 levels were measured by Western blotting of extracts from cells that were treated or not with roscovitine for 4 h. Also shown in the lower panel is the actin loading control. TRAP-220, thyroid receptor-associated protein; acetyl Lys 5, histone H3 acetyl lysine 5.