Figure 3.

Cordycepin suppresses inflammatory cytokine production and attenuates TLR4 and NFκB signal activation. Serum samples and liver tissues were harvested at 5 h after injected with GalN/LPS to mice. (a) Serum TNF-α, IL-1β, and IL-6 levels were assayed using ELISA method (n = 8). (b) Hepatic TNF-α, IL-1β, and IL-6 mRNA expressions were determined by qRT-PCR (n = 5). (c) F4/80 staining of liver sections were conducted to indicate the filtration of macrophages. Scale bars: 50 μm. (d) Hepatic MPO activity was measured according to manufacturer's instruction (n = 8). (e) Hepatic expression of TLR4, IκB, and p-IκB was analyzed by western blotting (n = 5). Each value is mean ± SEM. ^∗P < 0.05, ^∗∗P < 0.01, ^∗∗∗P < 0.001.