FIG. 5.

Electron micrograph and representative projection averages of negatively stained His₆-Prp19p. (Upper panel) Typical micrograph area of negatively stained His₆-Prp19p. Bar, 50 nm. (Lower panel) Twelve representative averages obtained by multireference alignment and classification of 9,725 His₆-Prp19p particle images. The averages reveal the variability of the position of the globular domains in relation to the central stalk. Side length of the average images is 40 nm. Number of particles in each projection average: panel 1, 50; panel 2, 51; panel 3, 35; panel 4, 30; panel 5, 35; panel 6, 39; panel 7, 41; panel 8, 22; panel 9, 64; panel 10, 7; panel 11, 46; panel 12, 22.