TABLE 1.
Strains used in this study
| Strain | Relevant genotype | Source |
|---|---|---|
| S. pombe strains | ||
| KGY246 | ade6-M210 ura4-D18 leu1-32 h− | Lab stock |
| KGY1318 | cwf8::cwf8-HA::Kanrade6-M216 leu1-32 ura4-D18 h+ | Lab stock |
| KGY1607 | cdc5::cdc5-myc13::Kanrade6-M210 leu1-32 ura4-D18 h− | Lab stock |
| KGY1608 | cwf8::cwf8-myc13::Kanrade6-M210 leu1-32 ura4-D18 h− | Lab stock |
| KGY2105 | cdc5::cdc5-HA::Kanrade6-M216 leu1-32 ura4-D18 h+ | Lab stock |
| KGY2108 | cdc5::cdc5-myc13::Kanr/cdc5::cdc5-HA::Kanrade6-M210/ade6-M216 leu1-32/leu1-32 ura4-D18/ura4-D18 h−/h+ | This study |
| KGY4476 | cwf8::cwf8-myc13::Kanr/cwf8::cwf8-HA::Kanrade6-M210/ade6-M216 leu1-32/leu1-32 ura4-D18/ura4-D18 h−/h+ | This study |
| KGY4985 | cdc5::cdc5-myc13::Kanrcwf8::cwf8-myc13::Kanrcwf7::cwf7-HA3::Kanrade6-M216 leu1-32 ura4-D18 h+ | This study |
| KGY4986 | cdc5::cdc5-TAP::Kanr/cdc5::cdc5-TAP::Kanrcwf8::cwf8-myc13::Kanr/cwf8::cwf8-HA3::Kanrade6-M210/ade6-M216 leu1-32/leu1-32 ura4-D18/ura4-D18 h−/h+ | This study |
| S. cerevisiae strains | ||
| KGY1811 | MATaprp19-1 ura3-52 lys2-801 ade2-101 leu2-Δ1 trp1-Δ1 | J. Abelson |
| KGY1294 | MATα CEF1::CEF1-HA3::Kanr | Lab stock |
| KGY1296 | PJ69-4A, MATa; trp1-190 leu2-3,112 ura3-52 his3-200 gal4 ΔLYS2 replaced by GAL1-HIS3 GAL2-ADE2, met2 replaced by GAL-LacZ | 16 |
| KGY1356 | MATα PRP19::PRP19-HA3::Kanr | Lab stock |