Table 3. High throughput sequencing read statistics in patient tumours and associated peripheral blood mononuclear cells (PBMCs).
| Patient | Tumour | WGS Reads | Percent Aligned | RNA-Seq Reads | Percent RNA Aligned | RNA Integrity Number (RIN) |
|---|---|---|---|---|---|---|
| 1 | 1 | NA | NA | 2.60E+09 | 99.99 | 8.1 |
| 1 | 2 | NA | NA | 3.24E+09 | 99.99 | 7.6 |
| 1 | 3 | 8.00E+08 | 95.35 | NA | NA | NA |
| 1 | 4 | NA | NA | 2.69E+09 | 99.99 | 8.4 |
| 1 | 6 | 6.62E+08 | 94.84 | NA | NA | NA |
| 1 | 9 | 6.85E+08 | 96.08 | NA | NA | NA |
| 1 | PBMC | 8.02E+08 | 93.37 | NA | NA | NA |
| 2 | 1 | 7.82E+08 | 96.05 | 2.60E+09 | 99.99 | 7.3 |
| 2 | 2 | NA | NA | 2.52E+09 | 99.99 | 7.1 |
| 2 | 4 | 7.99E+08 | 96.47 | NA | NA | NA |
| 2 | 6 | 7.62E+08 | 96.74 | NA | NA | NA |
| 2 | 8 | 7.45E+08 | 95.14 | NA | NA | NA |
| 2 | PBMC | 7.56E+08 | 95 | NA | NA | NA |
| 3 | 1 | 7.33E+08 | 95.45 | 2.63E+09 | 99.99 | 6.9 |
| 3 | 2 | 7.42E+08 | 95.63 | 2.43E+09 | 99.99 | 7.6 |
| 3 | 3 | 7.99E+08 | 95.49 | 2.98E+09 | 99.99 | 7.3 |
| 3 | 4 | 8.93E+08 | 94.64 | 2.98E+09 | 99.99 | 8 |
| 3 | PBMC | 5.77E+08 | 94.82 | NA | NA | NA |
| 4 | 1 | 7.94E+08 | 92.44 | 2.67E+09 | 99.99 | 6 |
| 4 | 4 | 7.37E+08 | 94.5 | 2.99E+09 | 99.99 | 6.7 |
| 4 | 9 | 7.61E+08 | 94.46 | 3.93E+09 | 99.99 | 7.9 |
| 4 | 10 | 7.41E+08 | 93.64 | 3.06E+09 | 99.99 | 6.8 |
| 4 | PBMC | 9.84E+08 | 95.43 | NA | NA | NA |
| 5 | 5 | 7.67E+08 | 95.69 | 2.08E+09 | 99.99 | 8.7 |
| 5 | 8 | NA | NA | 2.28E+09 | 99.99 | 8 |
| 5 | 11 | 7.21E+08 | 94.52 | NA | NA | NA |
| 5 | 12 | 6.82E+08 | 93.94 | NA | NA | NA |
| 5 | 13 | 7.45E+08 | 93.73 | NA | NA | NA |
| 5 | PBMC | 5.71E+08 | 94.97 | NA | NA | NA |
| 6 | 4 | 7.35E+08 | 95.97 | 2.45E+09 | 99.99 | 8.3 |
| 6 | 5 | 5.42E+08 | 94.56 | 2.71E+09 | 99.99 | 9 |
| 6 | 6 | 7.72E+08 | 94.89 | 2.69E+09 | 99.99 | 6.3 |
| 6 | 7 | 6.83E+08 | 95.07 | 2.39E+09 | 99.99 | 8.8 |
| 6 | PBMC | 7.81E+08 | 95.46 | NA | NA | NA |
| 8 | 1 | NA | NA | 2.39E+09 | 99.99 | 8.8 |
| 8 | 4 | 6.89E+08 | 95.72 | 2.15E+09 | 99.99 | 8.1 |
| 8 | 5 | 8.10E+08 | 94.9 | 2.38E+09 | 99.99 | 7.9 |
| 8 | 6 | 7.05E+08 | 95.37 | 2.56E+09 | 99.99 | 9 |
| 8 | 7 | 7.00E+08 | 92.64 | NA | NA | NA |
| 8 | PBMC | 6.90E+08 | 93.4 | NA | NA | NA |
| 9 | 1 | 7.17E+08 | 95.31 | 3.35E+09 | 99.99 | 6.9 |
| 9 | 6 | 8.16E+08 | 94.77 | 2.82E+09 | 99.99 | 6.8 |
| 9 | 7 | 7.97E+08 | 91.96 | NA | NA | NA |
| 9 | PBMC | 6.30E+08 | 93.77 | NA | NA | NA |
| 10 | 1 | 7.43E+08 | 94.67 | 2.34E+09 | 99.99 | 7.5 |
| 10 | 2 | 7.39E+08 | 93.63 | 2.51E+09 | 99.99 | 6.5 |
| 10 | 3 | 8.20E+08 | 95.46 | 2.68E+09 | 99.99 | 6.4 |
| 10 | 4 | 8.07E+08 | 93.78 | NA | NA | NA |
| 10 | PBMC | 7.57E+08 | 94.48 | NA | NA | NA |
| 11 | 1 | 8.42E+08 | 96.04 | NA | NA | NA |
| 11 | 2 | 6.88E+08 | 95.64 | NA | NA | NA |
| 11 | 3 | NA | NA | 2.20E+09 | 99.99 | 6.6 |
| 11 | 7 | 1.24E+09 | 94.37 | NA | NA | NA |
| 11 | 8 | 5.81E+08 | 95.08 | NA | NA | NA |
| 11 | 14 | NA | NA | NA | NA | NA |
| 11 | PBMC | 7.14E+08 | 94.61 | NA | NA | NA |
| 13 | 1 | 7.44E+08 | 94.52 | 2.57E+09 | 99.99 | 6 |
| 13 | 2 | 7.97E+08 | 94.96 | NA | NA | NA |
| 13 | 3 | 8.06E+08 | 94.77 | 2.79E+09 | 99.99 | 8.2 |
| 13 | 7 | 9.34E+08 | 92.81 | NA | NA | NA |