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. 2017 Apr 11;4:170043. doi: 10.1038/sdata.2017.43

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Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0 Metadata associated with this Data Descriptor is available at http://www.nature.com/sdata/ and is released under the CC0 waiver to maximize reuse.

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(a) Schematic diagram of the DRGFP system. I-SceI (yellow line) indicates the site cleaved by the meganuclease I-SceI. The I-SceI site is converted by HR into a new site for the enzyme BcgI (Bcg I red line). The first and the second cassettes are shown. Cassette II is not transcribed. (b) Schematic representation of the experimental protocol. HeLa DRGFP cells were transfected with (1) SCE+scrambledshRNA; (2) SCE+APEsh; (3) SCE+APEsh+APEwt (on the left). Recombinant (REC) and non-recombinant or NHEJ (NONREC) molecules were purified following each transfection. UNCUT represents control plasmid-transfected HeLa DRGFP cells. The arrows indicate the steps and the procedures undertaken in analyzing the DNA methylation data.