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. 2017 Apr 11;4:170043. doi: 10.1038/sdata.2017.43

Figure 6. Variation of methylation status and expression of the repaired GFP gene induced by manipulating BER (APE1) levels.

Figure 6

Cytofluorimetric analysis of DRGFP cells undergoing repair by HR. (a,b) show HR GFP cells 7 days after the DSB and HR, including a control without DSB. The upper panels show the gating strategy to visualize GFP positive cells. The lower panels show the distribution of GFP+ cells after HR. The horizontal lines indicate the fraction of low expressers (L) or high expressers (H), which have been extensively characterized1,3. (c,d) Show the gating and the distribution of L and H cells in GFP+ cells, in which APE1 levels were depleted or reconstituted 48 h after DSB.