FIG. 7.

Chromatin immunoprecipitation of the mim-1 enhancer region. Chromatin fragments prepared from BM2 cells or HD11 cells were subjected to immunoprecipitation using Myb-specific monoclonal antibody 5E11 (lanes 1), polyclonal antiserum against v-Myb (lanes 2), or polyclonal antiserum against C/EBPβ (lanes 3). Control precipitations were performed with normal rabbit serum (lanes 4) or without antibody (lanes 5). DNA isolated from the immunoprecipitates (lanes 1 to 6) or from the total chromatin preparation before immunoprecipitation (lanes 7) was analyzed by PCR using primers specific for the mim-1 enhancer. Lanes 6 show a PCR control to which no target DNA was added.