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. 2005 Jan;25(1):440–450. doi: 10.1128/MCB.25.1.440-450.2005

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Copyright © 2005, American Society for Microbiology

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FIG. 1. — Inhibition of meiotic DNA replication by moderate concentrations of glucose requires GRR1. (A) Late-log-phase cultures of a wild-type strain (SH1970) were transferred to Sp, Sp+0.5, or Sp+2. Spore formation was measured at the indicated times after transfer. (B) Late-log-phase cultures of SH1970 were transferred as described for panel A, and expression levels of IME1, IME2, and a control (DED1) transcript were measured at the indicated times by S1 analysis. (C) Late-log-phase cultures of a wild type (SH1970) and a grr1Δ mutant (SH1742) were transferred to Sp or Sp+0.5 medium, and DNA replication was measured at the indicated times after transfer. (D) Wild-type and grr1Δ late-log-phase cultures were transferred to Sp medium, and IME1, IME2, and control (DED1) transcript levels were analyzed at the indicated times as described for panel B.