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Spore packaging in the grr1Δ mutant. Mid-log-phase cultures of a grr1Δ mutant (SH1752) were transferred to Sp. After 72 h, nuclei were stained with DAPI and images were captured using Nomarski (left side) and fluorescence (right side) optics, with Nomarski optics on a Nikon TE2000-U with a ColorView imaging system and analySIS software (SIS). Arrows indicate unpackaged nuclei. DIC, differential interference contrast.
