TABLE 1.
S. cerevisiae strains used in this study
| Strain | Genotype | Source |
|---|---|---|
| SH777 | MATa/MATα ade2/ade2 can1:ADE2:CAN1/can1:ADE2:CAN1 his3-11,15/his3-11,15 leu2-3,112/ leu2-3,112 lys2 (3′Δ):HIS3:lys2(5′Δ)/LYS2 trp1-1/trp1-3′Δ ura3-1/ura3-1 | 32 |
| SH1740 | SH777 except grr1Δ::TRP1/grr1Δ::TRP1 | This study |
| SH1970 | SH777 except TRP1/TRP1 LEU2/LEU2 | 38 |
| SH1752 | SH777 except grr1Δ::TRP1/grr1Δ::TRP1 LEU2/LEU2 | 38 |
| SH2778 | SH777 except trp1/TRP1 | This study |
| SH2716 | MATα ade2 can1:ADE2:CAN1 his3-11,15 leu2-3,112 trp1-3′Δ ura3-1 grr1::URA3 w/GAL1-IME2::6XHA-TRP1 | S. Irniger |
| SH2811 | SH1970 containing pS652 (tetO-IME2-6XHA-URA3) | This study |
| SH2813 | SH1752 containing pS652 (tetO-IME2-6XHA-URA3) | This study |
| SH2877 | SH1970 containing pS669 [tetO-IME2(pestΔ)-6XHA-URA3] | This study |
| SH3219 | SH1740 containing pS714 (tetO-IME2-6XHA-LEU2) and pTer62 (cup1-mycUb.Ko-URA3) | This study |
| SH3204 | SH2778 containing pRS315 (LEU2) and pTer62 (Cup1-mycUb.Ko-URA3) | This study |
| SH2362 | SH1970 containing pRS316 (URA3) | This study |
| SH3195 | SH2778 containing pS714 (tetO-IME2-6XHA-LEU2) and pTer62 (Cup1-mycUb.Ko-URA3) | This study |
| W303 | MATa/MATα ade2/ade2 can1/can1 his3-11,15/his3-11,15 leu2-3,112/leu2-3,112 trp1-1/trp1-1 ura3-1/ura3-1 | |
| MTY1537 | W303 except cdc34-2/cdc34-2 | M. Tyers |
| MTY1538 | W303 except cdc53-1/cdc53-1 | M. Tyers |
| MTY1831 | MATaade2 can1-100 his3-11,15 leu2-3,112 trp1-1 ura3-1 sic1Δ | M. Tyers |
| SH3179 | MTY1537 with pS652 (tetO-IME2-6XHA-URA3) | This study |
| SH3181 | MTY1538 with pS652 (tetO-IME2-6XHA-URA3) | This study |
| SH3183 | W303 with pS652 (tetO-IME2-6XHA-URA3) | This study |
| SH2898 | SH777 except grr1Δ::TRP1/GRR1 LEU2/leu2-3,112 | This study |
| SH2896 | SH777 except grr1Δ::TRP1/grr1Δ::TRP1 LEU2/leu2-3,112 | This study |